Resources
Learn more about how you can accelerate your research with the WAVEsystem
-
TechNote 1
Sensitive kinetic analysis of small molecules binding to large drug targets
In this TechNote we show how the WAVEsystem can be used to accurately monitor binding kinetics of large target-to-analyte molecular weight (MW) ratios (>300:1), thanks to the expanded sensing field over which the Grating-Coupled Interferometry (GCI) technology measures for high sensitivity.
-
Application Page
Small Molecule Development
Real is crude
The Creoptix WAVEsystem provides a reliable environment for fragment-based drug discovery and small molecule screening, allowing for accurate off-rate kinetic analysis of crude reaction mixtures. With exquisite sensitivity, the ability to resolve extremely rapid dissociating kinetics, and innate compatibility with the high molecular weight ratios characteristic of most drug discovery programs, the Creoptix WAVEsystem improves fragment-based screening and kinetic analysis of small molecules to accelerate drug development.
-
TechNote 3
Highly accurate resolution of fast off-rates to significantly reduce false-positives
In this TechNote we show how the WAVEsystem can be used to accurately measure fast off-rate kinetics of weakly binding molecules. Thanks to a cartridge design that enables ultra-fast transition times of 150 ms, low potency hits can now be easily spotted for more successful drug discovery.
-
Publication
E-Life, 2018
W. Pitsawong, V. Buosi, R. Otten, R.V. Agafonov, A. Zorba, N. Kern, S. Kutter, G. Kern, R.A.P. Pádua, X. Meniche, D. Kern. "Dynamics of human protein kinase Aurora A linked to drug selectivity."
-
Publication
Scientific Reports, 2020
H. Jankovics, B. Kovacs, A. Saftics, T. Gerecsei, E. Tóth, I. Szekacs, F. Vonderviszt, R. Horvath. “Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers.”
-
TechNote 5
Highly sensitive analysis at low immobilization levels, without mass transport limitation
In this TechNote we show the remarkable sensitivity of the WAVEsystem. Thanks to the expanded sensing field over which the Grating-Coupled Interferometry (GCI) technology measures, high-resolution kinetic determination at even very low responses is possible, potentially reducing material costs significantly.
-
Virtual Seminar
Small molecules: binding kinetics no matter the size
- Screen, rank and characterize weak binders with off-rates up to 10 s-1.
- Study binding kinetics even at large analyte:ligand MW ratios (up to 1:1000).
- Experiment with crude mixtures, detergents and other additives without clogging.
-
Deck
Binding kinetics on fibrils with label-free, surface-based sensors
In collaboration with Istituto Mario Negri, Milano
Alzheimer’s disease (AD) is the most common form of dementia in older people. This neurodegenerative
disorder is characterized by the deposit of toxic b-amyloid plaques (amyloid fibrils) in the brain. b-Amyloid
can interact with many intracellular and extracellular molecules, hence the importance of characterizing both
binding affinity and kinetics of these interactions. With high sensitivity and the fastest off-rate resolution, the
Creoptix WAVEsystem enables kinetic studies of weak binding THT (with off-rates of 10 s-1) onto amyloid fibrils. -
Poster
Spotting the weakest binders
SLAS 2020
Reliably determine off-rates of up to 10s-1, starting with just a crude reaction mixture. Work with a wide variety of solvents - including acetonitrile and high concentrations of DMSO - and minimize the occurrence of false positives.
-
TechNote 7
Binding kinetics of a GPCR
Membrane proteins are notoriously difficult to study due to the requirement for a membrane-mimicking environment and their instability once extracted from a cellular membrane. Here we show the capability of the WAVE to measure the interaction of a peptide ligand agonist (NTA11) with a thermostabilized variant of the neurotensin receptor 1 (NTSR1) at highest resolution.
-
TechNote 11
Quick characterization of binding onto unpurified GPCRs
In this TechNote, we show how the WAVEsystem can be used for the kinetic analysis of G-protein binding onto detergent-solubilized, unpurified GPCRs. By shortening a typical 10-step purification process, this method requires significantly less material (30mL of cell culture) and less time (less than 1 hour), thereby offering an alternative for screening purposes at an early stage of the drug discovery process involving membrane proteins.
-
Application Page
Membrane Proteins
Real is native
Understanding the interaction between a membrane protein and a small molecule drug or monoclonal antibody therapeutic is fundamental to drug development since it provides valuable insight regarding drug potency and efficacy. Yet to fully understand such liaisons, it is vital that the measurement of binding kinetics between these molecules is reliable. With the sensitivity and versatility to measure analyte-membrane protein interactions in a wide variety of sample matrices, the Creoptix WAVEsystem enables more detailed investigation of membrane protein pharmacology, providing, for example, real-time drug binding affinities and label-free kinetics.
-
Interview
Transforming drug discovery on membrane proteins
Under the Microscope - Drug Target Review
In November 2017, leadXpro chose to strengthen its capabilities with the Creoptix® WAVEsystem. “We were looking for a label‑free, biophysical method to investigate binding affinity and kinetics of small molecules with challenging integral membrane proteins,” explained Michael Hennig, Chief Executive Officer of leadXpro...
-
Virtual Seminar
Binding kinetics onto membrane proteins
- Analyze multiprotein complexes and even larger particles like VLPs and crude membranes.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Poster
Keeping kinetics real
PEGS Europe 2019
Kinetic characterization of analytes binding to membrane proteins (GPCRs) embedded in virus-like particles (VLPs) and captured from crude cell membrane extracts (centrifugation-sonication). -
TechNote 10
Affinity and kinetics of antibodies in serum
In this TechNote we show how the WAVE system can be used to accurately determine kinetics in serum. Thanks to a no-clog microfluidic design, kinetic studies can be performed under conditions that mimic the native environment as closely as possible, enabling the translation of these results to the clinic.
-
TechNote 12
Antibody characterization from COVID-19 patient plasma binding to SARS-CoV-2 antigens
In this TechNote, we show how the WAVEsystem can be used for the characterization of binding affinity and kinetics of antibodies raised against SARSCoV-2 in clinical human blood plasma samples. With innovative disposable, no-clog microfluidics and high sensitivity, the WAVEsystem opens the door for antibody characterization directly from clinical blood plasma samples. The methods described here are also compatible with high concentrations of serum and plasma of SARS-CoV-2 patients’ samples.
-
Application Page
Serology
Real is (less)diluted
The Creoptix WAVEsystem combines superior resolution in signal and time with a crude-sample robustness normally only possible with plate-based assays. This provides dynamic, label free analysis of molecular interactions in a wide range of biofluids, providing full kinetic data that includes both affinity evaluation and highly accurate measurement of association and dissociation constants. Delivering a level of sensitivity superior to traditional Surface Plasmon Resonance (SPR) technologies, Creoptix’ proprietary Grating-Coupled Interferometry (GCI) technology is driving a new era of pharmacokinetics.
-
Poster
Full kinetic characterization of antibodies in biofluids
Immunotherapies and Innovations for Infectious Diseases 2019
Assess antibody profiling in undiluted serum, measure kinetics in plasma and quantify anti-drug-antibody (ADA) titers directly from blood serum. -
Webinar
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Webinar
Biochemical and biophysical characterization of biologics
In collaboration with Shawn Owen (University of Utah)
- Measuring kinetics in biofluids does provide valuable insights into a drug’s efficacy.
- How can I best characterize antibody drug conjugates using biochemical and biophysical techniques?
- Determination of level of payload and site of conjugation of ADCs using LC/MS.
- Thermal stability and binding affinity of ADCs characterization using DSC, IR, ITC and GCI.
- What is the Grating-Coupled Interferometry technology?
-
Podcast
Sybody® candidates compete for ACE2 binding onto SARS-CoV-2 Spike RBD
Rapid kinetic characterization for inhibition assays
Listen to Markus Seeger (University of Zurich) and Rony Nehmé (Creoptix) discuss binding affinities and selectivity of Sybody® candidates to the receptor-binding domain (RBD) region of SARS-CoV-2 Spike protein. Highlights:
- Why ELISA signals alone are not enough to predict potency in biologics development
- Why did Markus choose the WAVEsystem for the biomolecular interaction analysis at his lab?
- Why choosing the right candidates at the very early stages of research matters.
-
Poster
Augmented affinity in antibody-antigen interactions: impact on design of diagnostic assays
Presented by Mologic at PEGS Boston 2019
Anti-p24 antibody binding kinetics were measured using the Creoptix WAVEdelta system. The results of this study reveal a surprising enhancement in affinity due to a significant 21-fold increase in on-rate when anti-p24 mAb1 recognizes the anti-p24 mAb2-p24 complex.
-
Application Page
Biologics Development
Real is natural
The Creoptix® WAVEsystem enables researchers to study biologic drugs in a wide range of biofluids and other complex matrices. With superior sensitivity to traditional Surface Plasmon Resonance (SPR) technologies for more accurate measurement of binding affinity and binding kinetics, the Creoptix WAVEsystem is driving biologics development.
-
Publication
bioRxiv, 2020
J. D. Walter, C. A. J. Hutter, A. A. Garaeva, M. Scherer, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J. C. Earp, P. Egloff, M. Sorgenfrei, L. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. Zimmer, D. J. Slotboom, C. Paulino, P. Plattet, M. A. Seeger. “Highly potent bispecific sybodies neutralize SARS-CoV-2.”
-
Publication
Journal of Molecular Biology, 2019
F. Andres, L. Iamele, T. Meyer, J.C. Stüber, F. Kast, E. Gherardi, H.H. Niemann, A. Plückthun. "Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking."
-
TechNote 14
Shedding light on Sybody® candidates binding onto SARS-CoV-2 spike RBD
In this TechNote we show how the WAVE system can be used to understand the binding dynamics of Sybody® candidates to the receptor binding domain (RBD) of SARS-CoV-2, both provided by Linkster Therapeutics AG and the University of Zürich. With high sensitivity and robust microfluidics, the WAVEsystem is suitable for competition assays, confirming and enriching ELISA data. By providing a rapid kinetic characterization for inhibition assays, with ACE2 kindly provided by leadXpro, the WAVE is accelerating the development of therapeutics against SARS-CoV-2.
-
Publication
bioRxiv, 2020
J. D. Walter, C. A. .J. Hutter, I. Zimmermann, M. Wyss, P. Egloff, M. Sorgenfrei, L. M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, P. Plattet, M. A. Seeger. “Sybodies targeting the SARS-CoV-2 receptor-binding domain.”
-
Poster
A physicochemical approach to characterizing antibody-drug conjugates through stability into target validation
PEGSBoston Virtual 2020 - Waters & Collaborators
A panel of methods were used to establish comprehensive characterization of antibody-drug conjugates (ADCs), including liquid chromatography, mass spectrometry, size exclusion chromatography, microfluidic modulation spectroscopy, differential scanning calorimetry, grating-coupled interferometry (GCI) and isothermal titration calorimetry.
-
Webinar
Binding kinetics supporting peptide discovery
In collaboration with Pepscan
- Optimization of peptides for therapeutic and diagnostic purposes.
- Off-rate screening as a valuable alternative approach to small molecule fragments and peptide selection in drug discovery.
- GCI technology can be used as orthogonal validation technique for ELISA data.
- Measuring kinetics in biofluids provides valuable insights into a drug’s efficacy.
- CLIPS® as a powerful and bio-orthogonal peptide cross linking chemistry.
-
Webinar
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Webinar
Biochemical and biophysical characterization of biologics
In collaboration with Shawn Owen (University of Utah)
- Measuring kinetics in biofluids does provide valuable insights into a drug’s efficacy.
- How can I best characterize antibody drug conjugates using biochemical and biophysical techniques?
- Determination of level of payload and site of conjugation of ADCs using LC/MS.
- Thermal stability and binding affinity of ADCs characterization using DSC, IR, ITC and GCI.
- What is the Grating-Coupled Interferometry technology?
-
Poster
Real samples, real data
PEGS Europe 2019
Assess antibody profiling in undiluted biofluids for reliable kinetic profiling in conditions closer to real life. Study kinetics in aggregating proteins (fibrils). -
Webinar
Binding kinetics supporting peptide discovery
In collaboration with Pepscan
- Optimization of peptides for therapeutic and diagnostic purposes.
- Off-rate screening as a valuable alternative approach to small molecule fragments and peptide selection in drug discovery.
- GCI technology can be used as orthogonal validation technique for ELISA data.
- Measuring kinetics in biofluids provides valuable insights into a drug’s efficacy.
- CLIPS® as a powerful and bio-orthogonal peptide cross linking chemistry.
-
Publication
PNAS, 2020
S. Moussu, C. Broyart, G. Santos-Fernandez, S. Augustin, S. Wehrle, U. Grossniklaus, J. Santiago. "Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth."
-
Poster
Discovery of CLIPS binders to anti-TNFa mAb’s using phage display libraries
Presented by Pepscan
Here we report the use of a monocyclic phage library encoding for a fully randomized 10-
mer peptide sequence flanked by two cysteines that are linked via a CLIPSTM scaffold to identify peptide binders that bind to Infliximab. Binding assessed with the WAVEsystem. -
Webinar
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Publication
The EMBO Journal, 2019
K. Lau, R. Podolec, R. Chappuis, R. Ulm, M. Hothorn. "Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs."
-
Publication
PNAS, 2020
S. Okuda, S. Fujita, A. Moretti, U. Hohmann, V.G. Doblas, Y. Ma, A. Pfister, B. Brandt, N. Geldner, M. Hothorn. "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2."
-
Publication
PNAS, 2020
A. D. Crook, A. C. Willoughby, O. Hazak, S. Okuda, K. R. VanDerMolen, C. L. Soyars, P. Cattaneo, N. M. Clark, R. Sozzani, M. Hothorn, C. S. Hardtke, Z. L. Nimchuk. “BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots.”
-
Small Molecule/FBDD
Sensitive kinetic analysis of small molecules binding to large drug targets
In this TechNote we show how the WAVEsystem can be used to accurately monitor binding kinetics of large target-to-analyte molecular weight (MW) ratios (>300:1), thanks to the expanded sensing field over which the Grating-Coupled Interferometry (GCI) technology measures for high sensitivity.
-
Small Molecule/FBDD
Highly accurate resolution of fast off-rates to significantly reduce false-positives
In this TechNote we show how the WAVEsystem can be used to accurately measure fast off-rate kinetics of weakly binding molecules. Thanks to a cartridge design that enables ultra-fast transition times of 150 ms, low potency hits can now be easily spotted for more successful drug discovery.
-
Small Molecule/FBDD
Highly sensitive analysis at low immobilization levels, without mass transport limitation
In this TechNote we show the remarkable sensitivity of the WAVEsystem. Thanks to the expanded sensing field over which the Grating-Coupled Interferometry (GCI) technology measures, high-resolution kinetic determination at even very low responses is possible, potentially reducing material costs significantly.
-
Membrane Proteins
Binding kinetics of a GPCR
Membrane proteins are notoriously difficult to study due to the requirement for a membrane-mimicking environment and their instability once extracted from a cellular membrane. Here we show the capability of the WAVE to measure the interaction of a peptide ligand agonist (NTA11) with a thermostabilized variant of the neurotensin receptor 1 (NTSR1) at highest resolution.
-
Serology
Affinity and kinetics of antibodies in serum
In this TechNote we show how the WAVE system can be used to accurately determine kinetics in serum. Thanks to a no-clog microfluidic design, kinetic studies can be performed under conditions that mimic the native environment as closely as possible, enabling the translation of these results to the clinic.
-
Membrane Proteins
Quick characterization of binding onto unpurified GPCRs
In this TechNote, we show how the WAVEsystem can be used for the kinetic analysis of G-protein binding onto detergent-solubilized, unpurified GPCRs. By shortening a typical 10-step purification process, this method requires significantly less material (30mL of cell culture) and less time (less than 1 hour), thereby offering an alternative for screening purposes at an early stage of the drug discovery process involving membrane proteins.
-
Serology
Antibody characterization from COVID-19 patient plasma binding to SARS-CoV-2 antigens
In this TechNote, we show how the WAVEsystem can be used for the characterization of binding affinity and kinetics of antibodies raised against SARSCoV-2 in clinical human blood plasma samples. With innovative disposable, no-clog microfluidics and high sensitivity, the WAVEsystem opens the door for antibody characterization directly from clinical blood plasma samples. The methods described here are also compatible with high concentrations of serum and plasma of SARS-CoV-2 patients’ samples.
-
Biologics
Shedding light on Sybody® candidates binding onto SARS-CoV-2 spike RBD
In this TechNote we show how the WAVE system can be used to understand the binding dynamics of Sybody® candidates to the receptor binding domain (RBD) of SARS-CoV-2, both provided by Linkster Therapeutics AG and the University of Zürich. With high sensitivity and robust microfluidics, the WAVEsystem is suitable for competition assays, confirming and enriching ELISA data. By providing a rapid kinetic characterization for inhibition assays, with ACE2 kindly provided by leadXpro, the WAVE is accelerating the development of therapeutics against SARS-CoV-2.
-
Small Molecules/FBDD
Small molecules: binding kinetics no matter the size
- Screen, rank and characterize weak binders with off-rates up to 10 s-1.
- Study binding kinetics even at large analyte:ligand MW ratios (up to 1:1000).
- Experiment with crude mixtures, detergents and other additives without clogging.
-
Peptides
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Membrane Proteins
Binding kinetics onto membrane proteins
- Analyze multiprotein complexes and even larger particles like VLPs and crude membranes.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Serology
Biochemical and biophysical characterization of biologics
In collaboration with Shawn Owen (University of Utah)
- Measuring kinetics in biofluids does provide valuable insights into a drug’s efficacy.
- How can I best characterize antibody drug conjugates using biochemical and biophysical techniques?
- Determination of level of payload and site of conjugation of ADCs using LC/MS.
- Thermal stability and binding affinity of ADCs characterization using DSC, IR, ITC and GCI.
- What is the Grating-Coupled Interferometry technology?
-
Serology
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Peptides
Binding kinetics supporting peptide discovery
In collaboration with Pepscan
- Optimization of peptides for therapeutic and diagnostic purposes.
- Off-rate screening as a valuable alternative approach to small molecule fragments and peptide selection in drug discovery.
- GCI technology can be used as orthogonal validation technique for ELISA data.
- Measuring kinetics in biofluids provides valuable insights into a drug’s efficacy.
- CLIPS® as a powerful and bio-orthogonal peptide cross linking chemistry.
-
Biologics Development
Biochemical and biophysical characterization of biologics
In collaboration with Shawn Owen (University of Utah)
- Measuring kinetics in biofluids does provide valuable insights into a drug’s efficacy.
- How can I best characterize antibody drug conjugates using biochemical and biophysical techniques?
- Determination of level of payload and site of conjugation of ADCs using LC/MS.
- Thermal stability and binding affinity of ADCs characterization using DSC, IR, ITC and GCI.
- What is the Grating-Coupled Interferometry technology?
-
Biologics Development
Binding kinetics supporting peptide discovery
In collaboration with Pepscan
- Optimization of peptides for therapeutic and diagnostic purposes.
- Off-rate screening as a valuable alternative approach to small molecule fragments and peptide selection in drug discovery.
- GCI technology can be used as orthogonal validation technique for ELISA data.
- Measuring kinetics in biofluids provides valuable insights into a drug’s efficacy.
- CLIPS® as a powerful and bio-orthogonal peptide cross linking chemistry.
-
Biologics Development
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.
-
Plant Sciences
Probing signalling interactions by two methods: GCI and ITC
Biophysical methods applied to signalling interactions, including those in plant sciences by Kelvin Lau (Protein Production and Structure Core Facility (PTPSP, EPFL)
In this series of virtual seminar, we will walk you through the benefits of the WAVEsystem for plant sciences research. Expect to see high-quality kinetics in the toughest sample conditions! -
Small Molecules/FBDD
Scientific Reports, 2021
E.A. FitzGerald, M.T. Butko, P. Boronat, D. Cederfelt, M. Abramsson, H. Ludviksdottir, J.E. van Muijlwijk-Koezen, I.J.P. de Esch, D. Dobritzsch, T. Young, H. Danielson. “Discovery of fragments inducing conformational effects in dynamic proteins using a second-harmonic generation biosensor”
-
Small Molecules/FBDD
Scientific Reports, 2021
C. Hong, N.J. Byrne, B. Zamlynny, S. Tummala, L. Xiao, J.M. Shipman, A.T. Partridge, C. Minnick, M.J. Breslin, M.T. Rudd, et al. “Structures of active-state orexin receptor 2 rationalize peptide and small-molecule agonist recognition and receptor activation.”
-
Small Molecules/FBDD
Scientific Reports, 2020
H. Jankovics, B. Kovacs, A. Saftics, T. Gerecsei, E. Tóth, I. Szekacs, F. Vonderviszt, R. Horvath. “Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers.”
-
Plant Sciences
PNAS, 2020
A. D. Crook, A. C. Willoughby, O. Hazak, S. Okuda, K. R. VanDerMolen, C. L. Soyars, P. Cattaneo, N. M. Clark, R. Sozzani, M. Hothorn, C. S. Hardtke, Z. L. Nimchuk. “BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots.”
-
COVID-19 Research
bioRxiv, 2020
J. D. Walter, C. A. J. Hutter, A. A. Garaeva, M. Scherer, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J. C. Earp, P. Egloff, M. Sorgenfrei, L. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. Zimmer, D. J. Slotboom, C. Paulino, P. Plattet, M. A. Seeger. “Highly potent bispecific sybodies neutralize SARS-CoV-2.”
-
Biologics Development
bioRxiv, 2020
J. D. Walter, C. A. .J. Hutter, I. Zimmermann, M. Wyss, P. Egloff, M. Sorgenfrei, L. M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, P. Plattet, M. A. Seeger. “Sybodies targeting the SARS-CoV-2 receptor-binding domain.”
-
Peptides
PNAS, 2020
A. D. Crook, A. C. Willoughby, O. Hazak, S. Okuda, K. R. VanDerMolen, C. L. Soyars, P. Cattaneo, N. M. Clark, R. Sozzani, M. Hothorn, C. S. Hardtke, Z. L. Nimchuk. “BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots.”
-
COVID-19 Research
bioRxiv, 2020
J. D. Walter, C. A. .J. Hutter, I. Zimmermann, M. Wyss, P. Egloff, M. Sorgenfrei, L. M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, P. Plattet, M. A. Seeger. “Sybodies targeting the SARS-CoV-2 receptor-binding domain.”
-
Plant Sciences
Plant Physiology, 2020
P. Jimenez Sandoval, J. Santiago. “In vitro analytical approaches to study plant ligand-receptor interactions.”
-
Peptides
PNAS, 2020
S. Moussu, C. Broyart, G. Santos-Fernandez, S. Augustin, S. Wehrle, U. Grossniklaus, J. Santiago. "Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth."
-
Biologics Development
bioRxiv, 2020
J. D. Walter, C. A. J. Hutter, A. A. Garaeva, M. Scherer, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J. C. Earp, P. Egloff, M. Sorgenfrei, L. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. Zimmer, D. J. Slotboom, C. Paulino, P. Plattet, M. A. Seeger. “Highly potent bispecific sybodies neutralize SARS-CoV-2.”
-
Plant Sciences
Science, 2020
N. M. Doll, S. Royek, S. Fujita, S. Okuda, S. Chamot, A. Stintzi, T. Widiez, M. Hothorn, A. Schaller, N. Geldner. "A two-way molecular dialogue between embryo and endosperm is required for seed development."
-
Plant Sciences
PNAS, 2020
S. Okuda, S. Fujita, A. Moretti, U. Hohmann, V.G. Doblas, Y. Ma, A. Pfister, B. Brandt, N. Geldner, M. Hothorn. "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2."
-
Plant Sciences
PNAS, 2020
S. Moussu, C. Broyart, G. Santos-Fernandez, S. Augustin, S. Wehrle, U. Grossniklaus, J. Santiago. "Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth."
-
Protein-Protein Interactions
Analyst, 2019
B. Peter, A. Saftics, B. Kovacs, S. Kurunczi, R. Horvath. "Oxidation increases the binding of EGCG to serum albumin revealed by kinetic data from label-free optical biosensor with reference channel."
-
Plant Sciences
The EMBO Journal, 2019
K. Lau, R. Podolec, R. Chappuis, R. Ulm, M. Hothorn. "Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs."
-
Peptides
PNAS, 2020
S. Okuda, S. Fujita, A. Moretti, U. Hohmann, V.G. Doblas, Y. Ma, A. Pfister, B. Brandt, N. Geldner, M. Hothorn. "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2."
-
Peptides
The EMBO Journal, 2019
K. Lau, R. Podolec, R. Chappuis, R. Ulm, M. Hothorn. "Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs."
-
Biologics Development
Journal of Molecular Biology, 2019
F. Andres, L. Iamele, T. Meyer, J.C. Stüber, F. Kast, E. Gherardi, H.H. Niemann, A. Plückthun. "Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking."
-
Plant Sciences
Life Science Alliance Journal, 2019
L. Lorenzo-Orts, U. Hohmann, J. Zhu, M. Hothorn. "Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules."
-
Plant Sciences
Nature Plants, 2018
U. Hohmann, J. Nicolet, A. Moretti, L.A. Hothorn, M. Hothorn, "The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling."
-
Plant Sciences
PNAS, 2018
U. Hohmann, J. Santiago, J. Nicolet, V. Olsson, F. Spiga. L.A. Hothorn, M.A. Butenko, M. Hothorn, "Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors."
-
Small Molecules/FBDD
E-Life, 2018
W. Pitsawong, V. Buosi, R. Otten, R.V. Agafonov, A. Zorba, N. Kern, S. Kutter, G. Kern, R.A.P. Pádua, X. Meniche, D. Kern. "Dynamics of human protein kinase Aurora A linked to drug selectivity."
-
Real is crude
Small Molecule Development
The Creoptix WAVEsystem provides a reliable environment for fragment-based drug discovery and small molecule screening, allowing for accurate off-rate kinetic analysis of crude reaction mixtures. With exquisite sensitivity, the ability to resolve extremely rapid dissociating kinetics, and innate compatibility with the high molecular weight ratios characteristic of most drug discovery programs, the Creoptix WAVEsystem improves fragment-based screening and kinetic analysis of small molecules to accelerate drug development.
-
Real is native
Membrane Proteins
Understanding the interaction between a membrane protein and a small molecule drug or monoclonal antibody therapeutic is fundamental to drug development since it provides valuable insight regarding drug potency and efficacy. Yet to fully understand such liaisons, it is vital that the measurement of binding kinetics between these molecules is reliable. With the sensitivity and versatility to measure analyte-membrane protein interactions in a wide variety of sample matrices, the Creoptix WAVEsystem enables more detailed investigation of membrane protein pharmacology, providing, for example, real-time drug binding affinities and label-free kinetics.
-
Real is (less)diluted
Serology
The Creoptix WAVEsystem combines superior resolution in signal and time with a crude-sample robustness normally only possible with plate-based assays. This provides dynamic, label free analysis of molecular interactions in a wide range of biofluids, providing full kinetic data that includes both affinity evaluation and highly accurate measurement of association and dissociation constants. Delivering a level of sensitivity superior to traditional Surface Plasmon Resonance (SPR) technologies, Creoptix’ proprietary Grating-Coupled Interferometry (GCI) technology is driving a new era of pharmacokinetics.
-
Real is natural
Biologics Development
The Creoptix® WAVEsystem enables researchers to study biologic drugs in a wide range of biofluids and other complex matrices. With superior sensitivity to traditional Surface Plasmon Resonance (SPR) technologies for more accurate measurement of binding affinity and binding kinetics, the Creoptix WAVEsystem is driving biologics development.
-
Real is relevant
COVID-19 Research
SARS-CoV-2 proved challenging in ways current technologies were unable to address, limiting studies to extrapolations and inferences. It’s through innovation and technologies like the Creoptix® WAVEsystem that advance discovery and understanding. The drive to know more, study every angle, and make every measurement. With the ability to measure fast transitions in undiluted crude samples, all within a disposable cartridge, the WAVE makes challenging studies possible.
-
Real samples, real data
WAVEsystem
The WAVE of the future in kinetics
Confidently detect and quantify biological interactions in real-time, for high-quality kinetic data across a broader range of samples than traditional equipment.
The Creoptix WAVEsystem puts a breakthrough level of kinetics analysis at your fingertips by pushing the boundaries of affinity range and sample compatibility. The system’s exceptionally high data quality, sample compatibility and automated software facilitates drug discovery and enable new inroads into R&D.
-
A sensor chip for every need
WAVEchips
Robust microfluidics for a broad range of applications
- Explore any biomolecular interaction with a broad range of sensor chip surfaces for an all-in-one solution.
- Perform your experiments in biofluids (including serum and plasma), crude samples and uncommon solvents thanks to our no-clog microfluidics design for reduced maintenance costs.
- Exchange your WAVEchips to have fresh, clean microfluidics within minutes, without the need for special technical support.
- Enjoy the fastest and most synchronized sample transitions thanks to our parallel flow channels for high-quality kinetics.
-
Fast, intuitive, automated
WAVEcontrol
Workflow-based design offering both flexibility and functionality
- waveRAPID® – Kinetics in hours instead of days
RAPID stands for Repeated Analyte Pulses of Increasing Duration. This new way of measuring kinetics allows you to probe the interaction of interest using injections from a single well, with short pulses of increasing duration. - Direct Kinetics - Automated, objective way of evaluating data
We rely on robust statistical estimators to deliver an automated calculation of your kinetic parameters with a mathematically sound error analysis.
- waveRAPID® – Kinetics in hours instead of days
-
Smooth operations
WAVEcare
Carefree or basic support for high-quality kinetic data and unparalleled performance
The different WAVEcare programs ensure the continuous, high performance operation of your WAVEsystem. Choose the program that fits your needs.
- Carefree Program: for the regular user
Know what you are up to and plan your maintenance budget ahead of time - Basic Program: for the occasional user
Call us when you need us the most for a PM visit or a repair
- Carefree Program: for the regular user
-
Biologics Development
A physicochemical approach to characterizing antibody-drug conjugates through stability into target validation
PEGSBoston Virtual 2020 - Waters & Collaborators
A panel of methods were used to establish comprehensive characterization of antibody-drug conjugates (ADCs), including liquid chromatography, mass spectrometry, size exclusion chromatography, microfluidic modulation spectroscopy, differential scanning calorimetry, grating-coupled interferometry (GCI) and isothermal titration calorimetry.
-
Small Molecules/FBDD
Spotting the weakest binders
SLAS 2020
Reliably determine off-rates of up to 10s-1, starting with just a crude reaction mixture. Work with a wide variety of solvents - including acetonitrile and high concentrations of DMSO - and minimize the occurrence of false positives. -
Biologics Development
Augmented affinity in antibody-antigen interactions: impact on design of diagnostic assays
Presented by Mologic at PEGS Boston 2019
Anti-p24 antibody binding kinetics were measured using the Creoptix WAVEdelta system. The results of this study reveal a surprising enhancement in affinity due to a significant 21-fold increase in on-rate when anti-p24 mAb1 recognizes the anti-p24 mAb2-p24 complex.
-
Membrane Proteins
Keeping kinetics real
PEGS Europe 2019
Kinetic characterization of analytes binding to membrane proteins (GPCRs) embedded in virus-like particles (VLPs) and captured from crude cell membrane extracts (centrifugation-sonication). -
Peptides
Discovery of CLIPS binders to anti-TNFa mAb’s using phage display libraries
Presented by Pepscan
Here we report the use of a monocyclic phage library encoding for a fully randomized 10-
mer peptide sequence flanked by two cysteines that are linked via a CLIPSTM scaffold to identify peptide binders that bind to Infliximab. Binding assessed with the WAVEsystem. -
Biologics Development
Real samples, real data
PEGS Europe 2019
Assess antibody profiling in undiluted biofluids for reliable kinetic profiling in conditions closer to real life. Study kinetics in aggregating proteins (fibrils). -
Serology
Full kinetic characterization of antibodies in biofluids
Immunotherapies and Innovations for Infectious Diseases 2019
Assess antibody profiling in undiluted serum, measure kinetics in plasma and quantify anti-drug-antibody (ADA) titers directly from blood serum. -
Plant Sciences
GCI: a new method for label-free protein interaction studies
Plants, Peptides and Receptors in Málaga 2018
Here we characterize the interactions of various plant leucine-rich repeat receptor kinases (LRR-RKs) with their cognate ligands and SERK-family co-receptors using a label-free surface biosensor based on grating-coupled interferometry (GCI). Due to the comparably low amount of recombinant protein and analyte needed, GCI is an excellent alternative for experiments where key components are scarce. -
Biologics Development
Sybody® candidates compete for ACE2 binding onto SARS-CoV-2 Spike RBD
Rapid kinetic characterization for inhibition assays
Listen to Markus Seeger (University of Zurich) and Rony Nehmé (Creoptix) discuss binding affinities and selectivity of Sybody® candidates to the receptor-binding domain (RBD) region of SARS-CoV-2 Spike protein. Highlights:
- Why ELISA signals alone are not enough to predict potency in biologics development
- Why did Markus choose the WAVEsystem for the biomolecular interaction analysis at his lab?
- Why choosing the right candidates at the very early stages of research matters.
-
Grating-coupled interferometry
It's how we see light
Determine kinetics, affinity, and concentrations of interacting molecules with high sensitivity
Grating-Coupled Interferometry (GCI) is based on waveguide interferometry to determine kinetics, affinity, and concentrations of the interacting molecules. Similar to other optical label-free methods, such as surface plasmon resonance (SPR), the target (or ligand) is immobilized to the sensor surface and the analyte is flowed over the surface in what is called the bulk. Light is coupled to the surface creating an evanescent field. Interactions are detected by the resulting change in mass and thus a change in the refractive index.
-
No-clog microfluidic design
It's how we flow
Valveless microfluidics for fast transitions
The valveless design enables ultra-fast transition times of 150 msec for reliable determination of off-rates of 10 s–1 (half-life of 69 ms), enabling accurate off-rate kinetics of weakly binding fragments and low affinity antibodies.
No-clog microfluidics accommodates of a broad range of sample types to preserve activity and biological context, saving time from detrimental purification steps and clogging that takes other systems offline, including 100% whole blood or undiluted serum and plasma, cell supernatant, cell membrane preps, virus-like particles (VLPs), liposomes, viscous detergents, DMSO and acetonitrile.
-
Analyze Creoptix data in Genedata Screener
Genedata Ready-to-Run
Genedata and Creoptix offer a Ready-to-Run integration, allowing you to analyze data prepared with a Creoptix instrument in your Genedata Screener®.
The joint solution offers:
- Direct analysis of Grating-Coupled Interferometry in Genedata Screener.
- Sample data association across systems and characterization techniques.
- Reduced set-up and routine analysis times.
-
Small Molecules/FBDD
Binding kinetics on fibrils with label-free, surface-based sensors
In collaboration with Istituto Mario Negri, Milano
Alzheimer’s disease (AD) is the most common form of dementia in older people. This neurodegenerative
disorder is characterized by the deposit of toxic b-amyloid plaques (amyloid fibrils) in the brain. b-Amyloid
can interact with many intracellular and extracellular molecules, hence the importance of characterizing both
binding affinity and kinetics of these interactions. With high sensitivity and the fastest off-rate resolution, the
Creoptix WAVEsystem enables kinetic studies of weak binding THT (with off-rates of 10 s-1) onto amyloid fibrils. -
Keeping kinetics real
Specs WAVEsystem
Highlights
- waveRAPID - kinetics in hours instead of days (WAVEdelta only)
- Crude samples, harsh chemicals, and particles up to 1000 nm
- Self-contained disposable cartridge and small footprint
- Ultra-fast transition times of 150 ms with reliable determination of off-rates up to 10 s-1
- Superior signal-to-noise ratios (0.01 pg/mm2 at 1 Hz)
- Reliable kinetics and binding affinities (KD) from low pM to low mM with from signals below 1 pg/mm2
- Analyze large ligand‑to‑analyte molecular weight ratios up to >1000:1
- Up to 120 hours of unattended operation
-
Find the right WAVEchip for your application
WAVEchip Quickstart Guide
WAVEchips®- Innovative design and patented microfluidic cartridge to support crude samples, pathogenic samples, harsh solvents, and large particles up to 1000 nm normally only achieved with plate-based assays for kinetic analysis not possible before.
Explore any biomolecular interaction with a broad range of sensor chip surfaces for an all-in-one solution.
-
Make the most out of your WAVEsystem
Recommended vendors for consumables and reagents - EU
EU Edition
Everything our specialists recommend using when setting up experiments with your WAVEsystem:
- Well plates
- Reagents
- Vials & caps
- Foils
-
Make the most out of your WAVEsystem
Recommended vendors for consumables and reagents - US
US Edition
Everything our specialists recommend using when setting up experiments with your WAVEsystem:
- Well plates
- Reagents
- Vials & caps
- Foils
-
Membrane Proteins
Transforming drug discovery on membrane proteins
Under the Microscope - Drug Target Review
In November 2017, leadXpro chose to strengthen its capabilities with the Creoptix® WAVEsystem. “We were looking for a label‑free, biophysical method to investigate binding affinity and kinetics of small molecules with challenging integral membrane proteins,” explained Michael Hennig, Chief Executive Officer of leadXpro...
-
Poster
Augmented affinity in antibody-antigen interactions: impact on design of diagnostic assays
Presented by Mologic at PEGS Boston 2019
Anti-p24 antibody binding kinetics were measured using the Creoptix WAVEdelta system. The results of this study reveal a surprising enhancement in affinity due to a significant 21-fold increase in on-rate when anti-p24 mAb1 recognizes the anti-p24 mAb2-p24 complex.
-
Publication
Nature Plants, 2018
U. Hohmann, J. Nicolet, A. Moretti, L.A. Hothorn, M. Hothorn, "The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling."
-
Poster
GCI: a new method for label-free protein interaction studies
Plants, Peptides and Receptors in Málaga 2018
Here we characterize the interactions of various plant leucine-rich repeat receptor kinases (LRR-RKs) with their cognate ligands and SERK-family co-receptors using a label-free surface biosensor based on grating-coupled interferometry (GCI). Due to the comparably low amount of recombinant protein and analyte needed, GCI is an excellent alternative for experiments where key components are scarce. -
Publication
PNAS, 2020
S. Moussu, C. Broyart, G. Santos-Fernandez, S. Augustin, S. Wehrle, U. Grossniklaus, J. Santiago. "Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth."
-
Publication
Science, 2020
N. M. Doll, S. Royek, S. Fujita, S. Okuda, S. Chamot, A. Stintzi, T. Widiez, M. Hothorn, A. Schaller, N. Geldner. "A two-way molecular dialogue between embryo and endosperm is required for seed development."
-
Webinar
Probing signalling interactions by two methods: GCI and ITC
Biophysical methods applied to signalling interactions, including those in plant sciences by Kelvin Lau (Protein Production and Structure Core Facility (PTPSP, EPFL)
In this series of virtual seminar, we will walk you through the benefits of the WAVEsystem for plant sciences research. Expect to see high-quality kinetics in the toughest sample conditions! -
Publication
Plant Physiology, 2020
P. Jimenez Sandoval, J. Santiago. “In vitro analytical approaches to study plant ligand-receptor interactions.”
-
Publication
The EMBO Journal, 2019
K. Lau, R. Podolec, R. Chappuis, R. Ulm, M. Hothorn. "Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs."
-
Publication
Life Science Alliance Journal, 2019
L. Lorenzo-Orts, U. Hohmann, J. Zhu, M. Hothorn. "Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules."
-
Publication
PNAS, 2020
S. Okuda, S. Fujita, A. Moretti, U. Hohmann, V.G. Doblas, Y. Ma, A. Pfister, B. Brandt, N. Geldner, M. Hothorn. "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2."
-
Publication
PNAS, 2018
U. Hohmann, J. Santiago, J. Nicolet, V. Olsson, F. Spiga. L.A. Hothorn, M.A. Butenko, M. Hothorn, "Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors."
-
Publication
PNAS, 2020
A. D. Crook, A. C. Willoughby, O. Hazak, S. Okuda, K. R. VanDerMolen, C. L. Soyars, P. Cattaneo, N. M. Clark, R. Sozzani, M. Hothorn, C. S. Hardtke, Z. L. Nimchuk. “BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots.”
-
Podcast
Sybody® candidates compete for ACE2 binding onto SARS-CoV-2 Spike RBD
Rapid kinetic characterization for inhibition assays
Listen to Markus Seeger (University of Zurich) and Rony Nehmé (Creoptix) discuss binding affinities and selectivity of Sybody® candidates to the receptor-binding domain (RBD) region of SARS-CoV-2 Spike protein. Highlights:
- Why ELISA signals alone are not enough to predict potency in biologics development
- Why did Markus choose the WAVEsystem for the biomolecular interaction analysis at his lab?
- Why choosing the right candidates at the very early stages of research matters.
-
TechNote 12
Antibody characterization from COVID-19 patient plasma binding to SARS-CoV-2 antigens
In this TechNote, we show how the WAVEsystem can be used for the characterization of binding affinity and kinetics of antibodies raised against SARSCoV-2 in clinical human blood plasma samples. With innovative disposable, no-clog microfluidics and high sensitivity, the WAVEsystem opens the door for antibody characterization directly from clinical blood plasma samples. The methods described here are also compatible with high concentrations of serum and plasma of SARS-CoV-2 patients’ samples.
-
Application Page
COVID-19 Research
Real is relevant
SARS-CoV-2 proved challenging in ways current technologies were unable to address, limiting studies to extrapolations and inferences. It’s through innovation and technologies like the Creoptix® WAVEsystem that advance discovery and understanding. The drive to know more, study every angle, and make every measurement. With the ability to measure fast transitions in undiluted crude samples, all within a disposable cartridge, the WAVE makes challenging studies possible.
-
Publication
bioRxiv, 2020
J. D. Walter, C. A. J. Hutter, A. A. Garaeva, M. Scherer, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J. C. Earp, P. Egloff, M. Sorgenfrei, L. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. Zimmer, D. J. Slotboom, C. Paulino, P. Plattet, M. A. Seeger. “Highly potent bispecific sybodies neutralize SARS-CoV-2.”
-
TechNote 14
Shedding light on Sybody® candidates binding onto SARS-CoV-2 spike RBD
In this TechNote we show how the WAVE system can be used to understand the binding dynamics of Sybody® candidates to the receptor binding domain (RBD) of SARS-CoV-2, both provided by Linkster Therapeutics AG and the University of Zürich. With high sensitivity and robust microfluidics, the WAVEsystem is suitable for competition assays, confirming and enriching ELISA data. By providing a rapid kinetic characterization for inhibition assays, with ACE2 kindly provided by leadXpro, the WAVE is accelerating the development of therapeutics against SARS-CoV-2.
-
Publication
bioRxiv, 2020
J. D. Walter, C. A. .J. Hutter, I. Zimmermann, M. Wyss, P. Egloff, M. Sorgenfrei, L. M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, P. Plattet, M. A. Seeger. “Sybodies targeting the SARS-CoV-2 receptor-binding domain.”
-
Webinar
Early stage kinetics and validation of ELISA data
- High-quality kinetics in a wide range of crude samples ranging from peptides to membrane proteins and relevant clinical samples.
- WAVE goodbye to lengthy and laborious receptor purification procedures.
- Welcome contaminants and work with crude samples.