WAVEchip - Quickstart Guide

Find the right WAVEchip for your application

You are four steps away from finding the right WAVEchip for your application

Showing 10 of 10 chips
  • 4PCH WAVEchip

    Sensor surface functionalized with a high capacity polycarboxylate layer, suited for immobilization through amine coupling.

    TechNote 1 - Small Molecules

    Discover binding kinetics of large target:analyte MW ratios >300:1 using a 4PCH WAVEchip.

    TechNote 3 - Weakly Binding Molecules

    Accurately measure fast off-rate kinetics of weakly binding molecules with ultra-fast transition times of 150 ms using a 4PCH WAVEchip.

    TechNote 5 - Kinetics Below Rmax 1

    High-resolution kinetic determination at very low responses - potentially reducing material costs - using a 4PCH WAVEchip.

    Publication - E-Life, 2018

    "Dynamics of human protein kinase Aurora A linked to drug selectivity" - PCH WAVEchip

    Publication - PNAS, 2018

    "Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors" - PCH WAVEchip

    Publication - EMBO Journal, 2019

    "Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs" - PCH WAVEchip

    Publication - PNAS, 2020

    "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2" - 4PCH WAVEchip

    Publication - Scientific Reports, 2020

    "Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers" - 4PCH WAVEchip

    Q&A - WAVEchips

    How much ligand can you immobilize on the WAVEchips?

    The highest capacity chip in the Creoptix WAVEsystem portfolio, the 4PCH WAVEchip, can immobilize up to 35,000 pg/mm2 of BSA, but the experimental capacity depends on the exact ligand used.

  • 4PCH-STA WAVEchip

    Sensor surface functionalized with a high capacity polycarboxylate layer and pre-immobilized streptavidin, ideal for capturing biotinylated molecules.

    Publication - E-Life, 2018

    "Dynamics of human protein kinase Aurora A linked to drug selectivity" - PCH-STA WAVEchip

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    This depends on the ligand you are immobilizing / capturing. Biotinylated and His-tagged ligands can be captured on STA and NTA chips respectively.

  • 4PCH-NTA WAVEchip

    Sensor surface functionalized with a high capacity polycarboxylate layer and pre-immobilized NTA, ideal for capturing His-tagged molecules.

    TechNote 11 - Unpurified GPCRs

    Kinetic analysis of G-protein binding onto detergent-solubilized, unpurified GPCRs using a 4PCH-NTA WAVEchip. Less material and less time!

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    This depends on the ligand you are immobilizing / capturing. Biotinylated and His-tagged ligands can be captured on STA and NTA chips respectively.

    Q&A - WAVEchips

    How often can I use the WAVEchips?

    His-tagged ligand proteins captured on a Ni-NTA surface can be regenerated by EDTA and the ligand freshly re-captured multiple times. This means that such a chip can generally be used repeatedly, provided it remains inserted in the Creoptix WAVEsystem.

  • 4PCP WAVEchip

    Sensor surface functionalized with a thin polycarboxylate layer, suited for immobilization through amine coupling.

    TechNote 10 - Kinetics in Serum

    Kinetic studies under conditions that mimic native environment as closely as possible (serum) using 4PCP WAVEchip.

    TechNote 12 - Kinetics in Plasma

    Characterization of binding affinity and kinetics of antibodies raised against SARS-CoV-2 in clinical human blood plasma samples using 4PCP WAVEchip.

    Publication - Nature Plants, 2018

    "The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling" - PCP WAVEchip

    Publication - PNAS, 2018

    "Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors" - PCP WAVEchip

    Publication - Life Science Alliance Journal, 2019

    "Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules" - 4PCP WAVEchip

    Publication - Journal of Molecular Biology, 2019

    "Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking" - 4PCP WAVEchip

    Publication - PNAS, 2020

    "Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2" - 4PCP WAVEchip

    Publication - Science, 2020

    "A two-way molecular dialogue between embryo and endosperm is required for seed development" - 4PCP WAVEchip

    Publication - PNAS, 2020

    "Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth" - 4PCP WAVEchip

    Publication - PNAS, 2020

    "BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots" - 4PCP WAVEchip

  • 4PCP-STA WAVEchip

    Sensor surface functionalized with a thin polycarboxylate layer and pre-immobilized streptavidin, ideal for capturing biotinylated molecules.

    TechNote 14 - Orthogonal ELISA Validation

    Binding dynamics of Sybody® candidates to the receptor binding domain (RBD) of SARS-CoV-2 using 4PCP-STA WAVEchip.

    Publication - bioRxiv, 2020

    "Sybodies targeting the SARS-CoV-2 receptor-binding domain" - 4PCP-STA WAVEchip

    Publication - bioRxiv, 2020

    "Highly potent bispecific sybodies neutralize SARS-CoV-2" - 4PCP-STA WAVEchip

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    This depends on the ligand you are immobilizing / capturing. Biotinylated and His-tagged ligands can be captured on STA and NTA chips respectively.

    Poster - Crude Membrane Extracts

    Kinetic characterization of analytes binding to membrane proteins (GPCRs) captured from crude cell membrane extracts (centrifugation-sonication) using a 4PCP-STA WAVEchip.

    Rapid Kinetic Characterization for Inhibition Assays

    Drive biologics development with providing insights into binding affinities and selectivity using a 4PCP-STA WAVEchip.

  • 4PCP-NTA WAVEchip

    Sensor surface functionalized with a thin polycarboxylate layer and pre-immobilized NTA, ideal for capturing His-tagged molecules.

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    This depends on the ligand you are immobilizing / capturing. Biotinylated and His-tagged ligands can be captured on STA and NTA chips respectively.

    Q&A - WAVEchips

    How often can I use the WAVEchips?

    His-tagged ligand proteins captured on a Ni-NTA surface can be regenerated by EDTA and the ligand freshly re-captured multiple times. This means that such a chip can generally be used repeatedly, provided it remains inserted in the Creoptix WAVEsystem.

  • 4PCP-PAG WAVEchip

    Sensor surface functionalized with a thin polycarboxylate layer and pre-immobilized proteinA/G fusion, ideal for capturing the broadest range of IgG and FC-tagged proteins.

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    Generally speaking, IgG antibodies can be directly captured on PAG chips, while lipid-particles (e.g. liposomes) can be directly captured on LIP WAVEchips.

    Q&A - WAVEchips

    How often can I use the WAVEchips?

    Regenerations are possible with both Ni-NTA and Protein A/G chips, allowing for multiple uses.

  • 4PCP-LIP WAVEchip

    Sensor surface functionalized with a thin polycarboxylate layer and pre-immobilized lipophilic groups, ideal for capturing lipid-based particles or hydrophobic ligands.

    Publication - Analyst, 2019

    "Oxidation increases the binding of EGCG to serum albumin revealed by kinetic data from label-free optical biosensor with reference channel" - 4PCP-LIP WAVEchip

    Publication - Scientific Reports, 2020

    "Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers" - 4PCH-LIP WAVEchip

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    Generally speaking, IgG antibodies can be directly captured on PAG chips, while lipid-particles (e.g. liposomes) can be directly captured on LIP WAVEchips.

  • 4PCZ WAVEchip

    Low fouling sensor functionalized with a zwitterionic polymer bearing carboxylic acids and tertiary amines in similar densities. This sensor chip is suited for immobilization through amine coupling. Ideal for coupling proteins with low isoelectric points or polyanionic ligands.

    Deck - Kinetics on Fibrils

    Kinetic studies of weak binding THT (with off-rates of 10 s-1) onto amyloid fibrils using a 4PCZ WAVEchip.

    Poster - Real Samples, Real Data

    Experience outstanding resolution for very fast kinetics with off-rates up to 10 s-1 using a 4PCZ WAVEchip.

  • 4DXH-STA WAVEchip

    Sensor surface functionalized with a carboxymethyl-dextran layer. High capacity, sensor chip with pre-immobilized streptavidin, ideal for capturing biotinylated molecules.

    Q&A - WAVEchips

    How do I choose an appropriate immobilization/capture strategy for protein binding?

    This depends on the ligand you are immobilizing / capturing. Biotinylated and His-tagged ligands can be captured on STA and NTA chips respectively.

    TechNote 7 - Solubilized GPCRs

    Measure the interaction of a peptide ligand agonist (NTA11) with a thermostabilized variant of the neurotensin receptor 1 (NTSR1) at highest resolution using a DXH-STA WAVEchip.

  • It's how we flow

    Innovative, No-Clog Microfluidic Design

    No-clog microfluidics accommodates a broad range of sample types to preserve activity and biological context, saving time from detrimental purification steps and clogging that takes other systems offline.

    WAV Echip web landscape 3