Case Study on Weak Binders
Pushing the boundaries in pharmaceutical hit discovery with the novel waveRAPID® kinetic assay
Drug discovery high-throughput screening campaigns of thousands of compounds can produce a large number of hits showing potential interaction with the drug target. For the selection of those hits with a potential for further development, a full characterization of binding kinetics is required.
In a study conducted by an HTS drug discovery group of Idorsia Pharmaceuticals Ltd. is demonstrated the kinetic characterization of a large number of drugs hit compounds:
several experiments, binding kinetics of up to 146 hit compounds were
determined by waveRAPID, using pulsed injections of samples at a single
concentration onto the drug target previously immobilized on the sensor
- All analyzed compounds had been studied before on state-of-the-art SPR instrumentation, using traditional multi-cycle kinetic measurements
- Comparing the data obtained from the two technologies (GCI and SPR) revealed excellent correlation for the calculated dissociation constants (KD)
Understand how you can take only a few minutes to characterize each compound, and save significant time, materials – and costs by reading the following case study.